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申 请 号:CN201610575007
专利名称:一种微生物法转化雄甾烯二酮生产睾酮的方法
专利权人:江南大学
交易方式:协议
参考价格: 面议
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名称: | 一种微生物法转化雄甾烯二酮生产睾酮的方法 |
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申请号: | CN201610575007 |
申请日期: | 2016-07-20 |
申请人: | 江南大学 |
发明人: | 饶志明; 邵明龙; 张显; 杨套伟; 徐美娟 |
地址: | 214122 江苏省无锡市滨湖区蠡湖大道1800号 |
主分类号: | C12N15/53 |
分类号: | C12N15/53; C12N15/81; C12N1/19; C12P33/00; C12R1/645 |
主权项: | 一种经过密码子优化的17β-羟基类固醇脱氢酶基因,其特征在于,其核苷酸序列如SEQ?ID?NO.1所示。 |
摘要: | 一种重组Yarrowia?lipolytica高效生物转化雄甾烯二酮生产睾酮的方法,属于基因工程领域。该发明分别克隆了来源于人的17β?羟基类固醇脱氢酶和来源于酿酒酵母的葡萄糖脱氢酶,并将其在Y.lipolytica?Polh中过量共表达,为国内外首次通过构建NADPH辅酶循环系统在解脂耶氏酵母中高效转化雄甾烯二酮生产睾酮。对构建的基因工程菌株进行酶活力测定及胞内辅酶水平研究表明重组菌能够持续有效地转化雄甾烯二酮生产睾酮。在转化温度为37℃,转化pH为7.5,底物助溶剂为甲基化?β?环糊精,生物量为200g/L,底物浓度为5g/L。经过3次补料全细胞转化后,15g/L雄甾烯二酮转化为14.3g/L睾酮,为目前报道微生物转化生产睾酮的最高水平,为微生物工业化生产睾酮提供了基础。 |
start date | end date | title | description | icon | date limit | importance | link | modal |
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2016-10-12 | 公开 | js/timeglider/js/timeglider/icons/verticalLineGray.png | 50 | |||||
2016-11-09 | 实质审查的生效 | js/timeglider/js/timeglider/icons/verticalLineGray.png | 50 |
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#1 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/Application/Home/Controller/PraiseController.class.php(56): Yar_Client->theme_bar('get_bar_id', Array)
#2 [internal function]: Home\Controller\PraiseController->get_bar_id_by_from_id()
#3 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/App.class.php(173): ReflectionMethod->invoke(Object(Home\Controller\PraiseController))
#4 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/App.class.php(110): Think\App::invokeAction(Object(Home\Controller\PraiseController), 'get_bar_id_by_f...')
#5 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/App.class.php(204): Think\App::exec()
#6 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/Think.class.php(120): Think\App::run()
#7 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/ThinkPHP.php(97): Think\Think::start()
#8 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/index.php(27): require('/home/ftp/1520/...')
#9 {main}