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申 请 号:CN201810343821
专利名称:一种卷丹百合愈伤组织的诱导方法
专利权人:长江师范学院
交易方式:协议
参考价格: 面议
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名称: | 一种卷丹百合愈伤组织的诱导方法 |
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申请号: | CN201810343821 |
申请日期: | 2018-04-17 |
申请人: | 长江师范学院 |
发明人: | 符勇耀; 杨利平; 秦密; 高海洪 |
地址: | 408100 重庆市涪陵区聚贤大道16号 |
主分类号: | A01H4/00 |
分类号: | A01H4/00 |
主权项: | |
摘要: | 本发明公开了一种卷丹百合愈伤组织的诱导方法,包括以下步骤:将预处理并灭菌后的卷丹百合珠芽或鳞片接种于诱导培养基中,光照强度为700 lx‑900 lx,培养20‑30天,即得到卷丹百合的愈伤组织,所述诱导培养基为MS+1.00 mg/L 6‑BA+0.15 mg/L NAA+0.20 mg/L KT+0.05~0.1 mg/L 2, 4‑D,培养基pH值为5.8~6.2。通过6‑BA、NAA、KT和2, 4‑D成分含量及一定的光照之间的协同配伍作用,共同调节植物内源激素水平,从而诱导卷丹百合产生愈伤组织,诱导率达到71%。采用本发明方法仅20天可以有效诱导卷丹百合的愈伤组织形成,克服了其愈伤组织难于诱导的问题,为卷丹百合的无性繁殖、抗病育种以及分子生物学研究等奠定基础。 |
start date | end date | title | description | icon | date limit | importance | link | modal |
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2018-08-28 | 公开 | js/timeglider/js/timeglider/icons/verticalLineGray.png | 50 | |||||
2018-09-21 | 实质审查的生效 | js/timeglider/js/timeglider/icons/verticalLineGray.png | 50 |
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#1 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/Application/Home/Controller/PraiseController.class.php(56): Yar_Client->theme_bar('get_bar_id', Array)
#2 [internal function]: Home\Controller\PraiseController->get_bar_id_by_from_id()
#3 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/App.class.php(173): ReflectionMethod->invoke(Object(Home\Controller\PraiseController))
#4 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/App.class.php(110): Think\App::invokeAction(Object(Home\Controller\PraiseController), 'get_bar_id_by_f...')
#5 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/App.class.php(204): Think\App::exec()
#6 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/Think.class.php(120): Think\App::run()
#7 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/ThinkPHP.php(97): Think\Think::start()
#8 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/index.php(27): require('/home/ftp/1520/...')
#9 {main}