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申 请 号:CN201810595547
专利名称:一种无猝灭剂荧光探针CP-DNA及其制备方法、应用和检测微量Pb2+的方法
专利权人:岭南师范学院
交易方式:协议
参考价格: 面议
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名称: | 一种无猝灭剂荧光探针CP-DNA及其制备方法、应用和检测微量Pb2+的方法 |
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申请号: | CN201810595547 |
申请日期: | 2018-06-11 |
申请人: | 岭南师范学院 |
发明人: | 贾永梅; 冯宗财; 李志果; 余彪; 刘培炼 |
地址: | 524048 广东省湛江市赤坎区寸金路29号 |
主分类号: | G01N21/64 |
分类号: | G01N21/64; C12N15/11; C12Q1/6806; C09K11/06; C08G61/02 |
主权项: | |
摘要: | 本发明涉及一种无猝灭剂荧光探针CP‑DNA及其制备方法、应用和检测微量Pb2+的方法。所述探针的结构式如式(Ⅰ):,所述探针的最小分子量为11465,n>1。提供的探针为单标记荧光探针,将具有聚集诱导荧光猝灭特性的导电高分子CP与DNA探针分子共价键结合,构建无猝灭剂荧光探针CP‑DNA。在无Pb2+存在时,探针的疏水部分CP分子处于聚集状态,荧光猝灭;在有Pb2+存在时,探针的亲水部分DNA与Pb2+特异性结合,形成G‑四联体结构,其疏水部分CP分子处于分散状态,荧光释放,通过监测荧光信号变化,实现对Pb2+检测,灵敏度高,检测下限为10 ppM,可靠性高,成本低廉。 |
start date | end date | title | description | icon | date limit | importance | link | modal |
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2018-11-13 | 公开 | js/timeglider/js/timeglider/icons/verticalLineGray.png | 50 | |||||
2018-12-07 | 实质审查的生效 | js/timeglider/js/timeglider/icons/verticalLineGray.png | 50 |
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#1 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/Application/Home/Controller/PraiseController.class.php(56): Yar_Client->theme_bar('get_bar_id', Array)
#2 [internal function]: Home\Controller\PraiseController->get_bar_id_by_from_id()
#3 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/App.class.php(173): ReflectionMethod->invoke(Object(Home\Controller\PraiseController))
#4 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/App.class.php(110): Think\App::invokeAction(Object(Home\Controller\PraiseController), 'get_bar_id_by_f...')
#5 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/App.class.php(204): Think\App::exec()
#6 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/Think.class.php(120): Think\App::run()
#7 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/ThinkPHP.php(97): Think\Think::start()
#8 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/index.php(27): require('/home/ftp/1520/...')
#9 {main}