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申 请 号:CN201410053650
专利名称:酶活与热稳定性同时提高的脂肪氧合酶突变体及制备方法
专利权人:南京农业大学
交易方式:协议
参考价格: 面议
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名称: | 酶活与热稳定性同时提高的脂肪氧合酶突变体及制备方法 |
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申请号: | CN201410053650 |
申请日期: | 2014-02-18 |
申请人: | 南京农业大学 |
发明人: | 吕凤霞; 陆兆新; 郭芳芳; 张充; 别小妹; 赵海珍 |
地址: | 210095 江苏省南京市卫岗1号 |
主分类号: | C12N9/02 |
分类号: | C12N9/02; C12N15/53; C12N15/70 |
主权项: | 酶活与热稳定性同时提高的脂肪氧合酶突变体,其特征在于,其氨基酸残基序列如SEQ?ID?NO.1所示,其在一个氨基酸位点发生突变,即第305位氨基酸由天冬酰胺替换成了天冬氨酸。 |
摘要: | 本发明公开了一种酶活与热稳定性同时提高的脂肪氧合酶突变体及制备方法,利用定向进化技术对鱼腥藻脂肪氧合酶分子进行定向改造,经多轮易错PCR和DNAShuffling,获得了酶活与热稳定性同时提高的脂肪氧合酶突变体,酶活力为21420U/mL,比野生型提高了2.17倍,其在50℃的半衰期t1/2提高了1.9倍。利用此策略可以显著提高脂肪氧合酶的酶活和热稳定性,解决了脂肪氧合酶活低和热稳定性较差的问题,为其工业化生产提供了基础。改造后的脂肪氧合酶更适用于工业生产和应用。 |
start date | end date | title | description | icon | date limit | importance | link | modal |
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2014-05-28 | 公开 | js/timeglider/js/timeglider/icons/verticalLineGray.png | 50 | |||||
2014-06-25 | 实质审查的生效 | js/timeglider/js/timeglider/icons/verticalLineGray.png | 50 | |||||
2016-04-20 | 授权 | js/timeglider/js/timeglider/icons/verticalLineGray.png | 50 |
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#1 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/Application/Home/Controller/PraiseController.class.php(56): Yar_Client->theme_bar('get_bar_id', Array)
#2 [internal function]: Home\Controller\PraiseController->get_bar_id_by_from_id()
#3 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/App.class.php(173): ReflectionMethod->invoke(Object(Home\Controller\PraiseController))
#4 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/App.class.php(110): Think\App::invokeAction(Object(Home\Controller\PraiseController), 'get_bar_id_by_f...')
#5 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/App.class.php(204): Think\App::exec()
#6 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/Library/Think/Think.class.php(120): Think\App::run()
#7 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/ThinkPHP/ThinkPHP.php(97): Think\Think::start()
#8 /home/ftp/1520/ipa361_com-20150420-MrH/ipa361.com/mapi/index.php(27): require('/home/ftp/1520/...')
#9 {main}